pDR195

貨號 名稱 規格 小包裝 大包裝
P1398 pDR195 質粒干粉 QQ詢價 QQ詢價
親愛的科研工作者,感謝您信任淼靈質粒平臺,我們致力于為大家分享質優價適的質粒,我們的各批次質粒干粉在發貨前均經過嚴格的多重驗證,您收到后請先轉化,再挑取單克隆擴增,萬一存在質量問題,請及時通知我們退換。如本頁面質粒信息不全,請及時聯系所在區域負責人QQ加急上傳。
別名: Plasmid #36028
啟動子: PMA1 promoter
復制子: pUC ori
終止子: ADH1 terminator
質粒分類: 酵母細胞質粒;酵母表達質粒
質粒大小: 6305bp
原核抗性: Amp
真核抗性: URA3
克隆菌株: DH5a
培養條件: 37度
表達宿主: 釀酒酵母
5'測序引物: PMA1-F:CGTTAATAATTAATTAATTGG
3'測序引物: ADH1ter-R:GAGTCACTTTAAAATTTGTATACAC
備注: 高拷貝
質粒宿主: 酵母菌
質粒用途: 蛋白表達
片段類型:
片段物種:
原核抗性: Amp
真核抗性: URA3
熒光標記:
         pDR195質粒是一個釀酒酵母表達載體,PMA1啟動子驅動目的基因的表達,其參考文獻是NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis. 
LOCUS       Exported                6305 bp ds-DNA     circular SYN 25-AUG-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2017 from MiaoLingPlasmid
            http://www.oqrbmj.live
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     510..530
                     /label=PMA1-F
     misc_feature    596..614
                     /label=MCS
     terminator      751..938
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(806..830)
                     /label=ADH1ter-R
     rep_origin      1402..2748
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(2859..3662)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3663..3878)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        3989..4093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4094..4954
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5125..5713
                     /direction=RIGHT
                     /label=pUC ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6127..595
                     /label=PMA1 promoter
ORIGIN
        1 ggagaaactc ctgcgagaat ttcgtaaact cgcgcgcatt gcatctgtat ttcctaatgc
       61 ggcacttcca ggcctcgatc gagaccgttt atccattgct tttttgttgt ctttttccct
      121 cgttcacaga aagtctgaag aagctatagt agaactatga gctttttttg tttctgtttt
      181 cctttttttt ttttttacct ctgtggaaat tgttactctc acactcttta gttcgtttgt
      241 ttgttttgtt tattccaatt atgaccggtg acgaaacgtg gtcgatggtg ggtaccgctt
      301 atgctcccct ccattagttt cgattatata aaaaggccaa atattgtatt attttcaaat
      361 gtcctatcat tatcgtctaa catctaattt ctcttaaatt ttttctcttt ctttcctata
      421 acaccaatag tgaaaatctt tttttcttct atatctacaa aaactttttt tttctatcaa
      481 cctcgttgat aaattttttc tttaacaatc gttaataatt aattaattgg aaaataacca
      541 ttttttctct cttttataca cacattcaaa agaaagaaaa aaaatatacc ccagcctcga
      601 gcggccgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
      661 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
      721 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
      781 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      841 aaacgaraat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      901 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca ctggccgtcg
      961 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1021 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1081 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     1141 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat
     1201 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
     1261 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
     1321 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
     1381 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
     1441 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     1501 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     1561 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     1621 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     1681 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     1741 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     1801 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     1861 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     1921 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     1981 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     2041 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     2101 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     2161 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     2221 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     2281 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     2341 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact
     2401 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     2461 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     2521 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     2581 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     2641 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     2701 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata
     2761 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt
     2821 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     2881 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     2941 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     3001 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     3061 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     3121 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     3181 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     3241 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     3301 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     3361 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     3421 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     3481 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     3541 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     3601 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     3661 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg
     3721 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     3781 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     3841 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa
     3901 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3961 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4021 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4081 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4141 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4201 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4261 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4321 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4381 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4441 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4501 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4561 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4621 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4681 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4741 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4801 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4861 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4921 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4981 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5041 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5461 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5521 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5581 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5641 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5701 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5761 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5821 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5881 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5941 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6001 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6061 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6121 ttacgccaag cttcctgaaa cggagaaaca taaacaggca ttgctgggat cacccataca
     6181 tcactctgtt ttgcctgacc ttttccggta atttgaaaac aaacccggtc tcgaagcgga
     6241 gatccggcga taattaccgc agaaataaac ccatacacga gacgtagaac cagccgcaca
     6301 tggcc
//
 
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